ZRB1354

Anti-Aldolase C Antibody, clone 3B18, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinonimo/i: Brain-type aldolase, EC:4.1.2.13, Fructose-bisphosphate aldolase C, Zebrin II
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit (recombinant)
• Livello qualitativo: 200
• Ricombinante: expressed in HEK 293 cells
• Coniugato: unconjugated
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 3B18, monoclonal; recombinant monoclonal
• Nome Commerciale: ZooMAb^® learn more
• Forma fisica: lyophilized
• PM: calculated mol wt 39.46 kDa; observed mol wt ~39 kDa
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): mouse
• Confezionamento: antibody small pack of 25 μL
• Caratteristiche più verdi: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Convalida avanzata: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• tecniche: immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotipo: IgG
• N° accesso UniProt: P09972
• Categoria alternativa più verde: Aligned,
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ALDOC(230)

Descrizione

Descrizione generale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificità

Clone 3B18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Aldolase C. It targets an epitope within 22 amino acids from the C-terminal region.

Immunogeno

KLH-conjugated liner peptide corresponding to 22 amino acids from the C-terminal region of human Aldolase C.

Applicazioni

Anti-Aldolase C, clone 3B18 ZooMAb, Cat. No. ZRB1354, is a recombinant Rabbit monoclonal antibody that targets Aldolase C and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Aldolase C in human brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Aldolase C in human cerebellum and human cerebral cortex tissue sections.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Aldolase C in human cerebellum tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrizione del bersaglio

Fructose-bisphosphate aldolase C (UniProt: P09972; also known as EC:4.1.2.13, Brain-type aldolase) is encoded by the ALDOC (also known as ALDC) gene (Gene ID: 230) in human. Aldolases are glycolytic enzymes that catalyze the reversible conversion of fructose 1-6-diphosphate to glyceraldehyde 3-phosphate and dihydroxy-acetone phosphate via the glycolytic pathway. Within the cell, aldolase is localized in both the cytoplasm and nucleus. In vertebrates, three forms of this aldolases have been described, aldolase A in muscle, aldolase B in liver, and aldolase C in brain. Aldolase C is a homotetrameric enzyme that is expressed mainly in cerebellum and retina. Its kinetic properties are somewhat intermediate between those of the A and B isoforms. In fetal tissues only low levels of aldolase C have been detected. It serves as a useful marker for the study of cerebellar compartmentalization, due to its specific expression in distinct subpopulations of cerebellar Purkinje cells. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Fujita. H., et al. (2014). PLoS ONE 9(1): e86679; Waltheer, EU., et al. (1998). Proc. Natl. Acad. Sci. USA. 95(5); 2615-2620).

Stato fisico

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Ricostituzione

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stoccaggio e stabilità

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Note legali

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable