ZRB1315

Anti-VDAC1 Antibody, clone 3M22, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinonimo/i: Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC-1, Voltage-dependent anion-selective channel protein 1 1, hVDAC1
• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: rabbit (recombinant)
• Livello qualitativo: 200
• Ricombinante: expressed in HEK 293 cells
• Coniugato: unconjugated
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 3M22, monoclonal; recombinant monoclonal
• Nome Commerciale: ZooMAb^® learn more
• Forma fisica: lyophilized
• PM: calculated mol wt 30.77 kDa; observed mol wt ~35 kDa
• Reattività contro le specie: mouse, human, rat
• Confezionamento: antibody small pack of 25 μL
• Caratteristiche più verdi: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Convalida avanzata: recombinant expression; Learn more about Antibody Enhanced Validation
• tecniche: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotipo: IgG
• N° accesso UniProt: P21796
• Categoria alternativa più verde: , Aligned
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Vdac1(22333)

Descrizione

Descrizione generale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificità

Clone 3M22 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Voltage-dependent anion-selective channel protein 1 (VDAC1). It targets an epitope within the C-terminal half.

Immunogeno

His-tagged recombinant fragment corresponding to 150 amino acids from the C-terminal half of human Voltage-dependent anion-selective channel protein 1 (VDAC1).

Applicazioni

Anti-VDAC1, clone 3M22 ZooMAb, Cat. No. ZRB1315, is a recombinant rabbit monoclonal antibody that detects Voltage-dependent anion-selective channel protein 1 (VDAC1) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected VDAC1 in HEK293, NIH3T3, and C6 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VDAC1 in HeLa, A431, HUVEC, NIH 3T3, MCF-7, NTERA-2 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected VDAC1 in human kidney and human heart tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrizione del bersaglio

Voltage-dependent anion-selective channel protein 1 (UniProt: P21796; also known as VDAC-1, hVDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM) is encoded by the VDAC1 (also known as VDAC) gene (Gene ID: 7416) in human. VDAC1 is a multi-pass membrane protein that is expressed on the outer mitochondrial membrane (OMM). It is one of the most abundant proteins on OMM. It serves as the gatekeeper for the passages of metabolites, nucleotides, and ions and with its interaction with Bcl-2 it plays a crucial role in regulating apoptosis. VDAC1 consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. Its helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity. VDAC1 forms a channel through the mitochondrial membrane and is involved in small molecule diffusion, cell volume regulation and apoptosis. It also participates in the formation of the permeability transition pore complex that is responsible for the release of mitochondrial products that triggers apoptosis. VDAC1 adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is shown to be cation-selective and can transport K+, Na+ and Ca2+. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Camara, AKS et al. (2017). Front. Physiol. 8; 460).

Stato fisico

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Ricostituzione

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stoccaggio e stabilità

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Note legali

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable