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Z374911

PCR multiwell plates

size 384 wells, polypropylene, skirt, non-sterile

Sinonimo/i:

384 multiwell PCR plate, 384 well PCR microplate, 384 well PCR plate, 384 well microtiter plate

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About This Item

Codice UNSPSC:
41106300
NACRES:
NB.22

Materiali

colorless polypropylene
polypropylene

Sterilità

non-sterile
non-sterile

Caratteristiche

skirt

Confezionamento

case of 50 ea

Produttore/marchio commerciale

Sorenson 39620

tecniche

PCR: suitable

Misura

384 wells

Volume pozzetto

40 μL

Volume di lavoro pozzetto

25 μL

Compatibilità

suitable for (PCR, RT-PCR or DNA purification applications)

applicazioni

agriculture

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Descrizione generale

384 well PCR plates are skirted for compatibility with automation systems. Wells have raised rims to ensure contact with sealing film and reduce evaporation. Each well has a capacity of 40 μL and a working volume of 25 μL.
A rigid top plate (included) minimizes plate distortion, assures a dependable fit with the PCR thermal cycler, and allows for a leak-proof seal with micro-mats or cap strips. Each well has a maximum capacity of 200 μL.

Caratteristiche e vantaggi

  • Virgin polypropylene
  • Fully autoclavable
  • Certified DNase- and RNase-free
  • Wells have thin walls for rapid temperature equilibration and reduced cycle time

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Protocolli

Reverse Transcription Protocol (One-step Probe Detection)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

dNTP Mediated Hot Start PCR Protocol

Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.

qPCR Gene Expression Using Probe Detection

The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.

qPCR Using a Single Detection Probe Protocol

A protocol that can be used as a basic template for qPCR incorporating a detection probe that is specific to a single target. In these reactions, primers and probe are included at a final concentration of 200 nM and are run using LuminoCt® ReadyMix™.

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