Passa al contenuto
Merck
Tutte le immagini(1)

Documenti fondamentali

Visualizza tutta la documentazione

T3038

Sigma-Aldrich

Trizma® hydrochloride solution

1 M, BioReagent, for molecular biology

Sinonimo/i:

Tris hydrochloride solution

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Numero MDL:
MFCD00012590
Codice UNSPSC:
12161700
ID PubChem:
24900102
NACRES:
NA.25

Grado

for molecular biology
for molecular biology

Livello qualitativo

200

Sterilità

0.2 μm filtered

Nome Commerciale

BioReagent

Forma fisica

solution

Concentrazione

1 M

Impurezze

DNase, RNase, NICKase and protease, none detected

pH

8.0

applicazioni

agriculture

Stringa SMILE

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
QKNYBSVHEMOAJP-UHFFFAOYSA-N

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Descrizione generale

Trizma® hydrochloride solution is a useful biological buffer.

Applicazioni

Trizma® hydrochloride solution may be used in the following studies:
  • As buffer for the 2-D electrophoresis of rat fibroblast cell.
  • As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).
  • Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Altre note

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Note legali

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Slide 1 of 7

1 of 7

Trizma® hydrochloride solution pH 7.4, BioPerformance Certified, 1 M, suitable for cell culture

Sigma-Aldrich

T2194

Trizma® hydrochloride solution

Tris-HCl 1 M, pH 6,5 1M Tris-HCl, pH 6.5.

Sigma-Aldrich

20-160

Tris-HCl 1 M, pH 6,5

Tetramethylammonium chloride solution for molecular biology

Sigma-Aldrich

T3411

Tetramethylammonium chloride solution

Trizma® cloridrato reagent grade, ≥99.0% (titration), crystalline

Sigma-Aldrich

T3253

Trizma® cloridrato

Tris(idrossimetil)aminometano Sterile solution. Suitable for DNA and RNA applications. Tris buffer is useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

Millipore

648314

Tris(idrossimetil)aminometano

Potassio cloruro BioUltra, for molecular biology, ~1 M in H2O

Sigma-Aldrich

60142

Potassio cloruro

Sodio cloruro 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture

Sigma-Aldrich

S5150

Sodio cloruro

Lisa W von Friesen et al.
Marine pollution bulletin, 142, 129-134 (2019-06-25)
Standardized methods for the digestion of biota for microplastic analysis are currently lacking. Chemical methods can be effective, but can also cause damage to some polymers. Enzymatic methods are known to be gentler, but often laborious, expensive and time consuming.
P R Langer et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(11), 6633-6637 (1981-11-01)
Analogs of dUTP and UTP that contain a biotin molecule covalently bound to the C-5 position of the pyrimidine ring through an allylamine linker arm have been synthesized. These biotin-labeled nucleotides are efficient substrates for a variety of DNA and
M G Murray et al.
Nucleic acids research, 8(19), 4321-4325 (1980-10-10)
A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. The procedure yields total cellular DNA
Emilie M F Kallenbach et al.
The Science of the total environment, 786, 147455-147455 (2021-05-09)
Chitinaceous organisms have been found to ingest microplastic; however, a standardised, validated, and time- and cost-efficient method for dissolving these organisms without affecting microplastic particles is still required. This study tested four protocols for dissolving organisms with a chitin exoskeleton:
F Gharahdaghi et al.
Electrophoresis, 20(3), 601-605 (1999-04-27)
Mass spectrometry is a powerful technique for the identification of proteins at nanogram quantities. However, some degree of sample preparation prior to mass spectrometry is required, and silver-stained protein gel samples are most problematic. Here we report our strategy to
Visualizza tutti i documenti correlati

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.