SRP8001

Calreticulin human

recombinant, expressed in E. coli, His tagged, >90% (SDS-PAGE)

• Sinonimo/i: CRP55, Calregulin, ERp60, Endoplasmic reticulum resident protein 60, grp60
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in E. coli
• Tag: His tagged
• Saggio: >90% (SDS-PAGE)
• Stato: liquid
• PM: ~55 kDa by SDS-PAGE
• Confezionamento: pkg of 10 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• tecniche: calcium flux assay: suitable
• Impurezze: <1 EU/μg endotoxin, tested
• N° accesso UniProt: P27797
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... CALR(811)

Descrizione

Descrizione generale

Calreticulin (CALR) is expressed in the endoplasmic reticulum. The gene encoding it is localized on human chromosome 19p13.13.

Applicazioni

Human calreticulin has been used in a study to determine the effects of uncoupling protein 4 (UCP4) on the expression of proteins in the store-operated Ca²⁺ entry pathway.

Azioni biochim/fisiol

Calreticulin is involved in regulation of intracellular Ca²⁺ homoeostasis and ER Ca²⁺ capacity. It constitutes together with calnexin and ERp57 the ′calreticulin/calnexin cycle′ that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule. Recently, Calreticulin was shown to enhance the merger of macrophages and tumor cells, increasing phagocytosis.

Stato fisico

Solution in 55 mM TRIS-HCl, pH 8.2, containing 150 mM NaCl.

Altre note

Human calreticulin (aa18-417) is fused at the C-terminus to a His-tag.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable