SRP6414

Cathepsin B human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Sinonimo/i: APPS, CPSB, CTSB
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in HEK 293 cells
• Tag: 6-His tagged (C-terminus)
• Saggio: ≥95% (SDS-PAGE)
• Forma fisica: lyophilized
• PM: calculated mol wt 29 kDa (Mature); calculated mol wt 36.7 kDa (Pro); observed mol wt 34 kDa; observed mol wt 43_kDaand34 kDa
• Confezionamento: pkg of 10 μg
• Impurezze: <1 EU/μg endotoxin (LAL test)
• N° accesso UniProt: P07858
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... CTSB(1508)

Descrizione

Descrizione generale

Cathepsin B (CTSB) also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds; preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L); In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.

DTT-reduced. Protein migrates due to glycosylation. Arg18 (pro) or Phe74 (mature) is the predicted N-terminal.

Stato fisico

Lyophilized from 0.22 μm filtered solution in 50 mM Tris-HCl and 150 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Ricostituzione

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable