SRP5355

MYT1, GST tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Sinonimo/i: DKFZp547K1610, FLJ20093, PKMYT1
• Numero CAS: 187414-13-7
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in baculovirus infected Sf9 cells
• Saggio: ≥70% (SDS-PAGE)
• Forma fisica: buffered aqueous glycerol solution
• PM: ~93 kDa
• N° accesso NCBI: NM_004203
• applicazioni: cell analysis
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... PKMYT1(9088)

Descrizione

Descrizione generale

The PKMYT1 (protein kinase, membrane associated tyrosine/threonine 1) gene is differentially expressed between fetal liver and postnatal liver. This gene is mapped to human chromosome 16p13.3. It is also known to be localized to immature oocytes. MYT1 (myelin transcription factor 1) is a member of a family of neural specific, zinc finger-containing DNA-binding proteins. MYT1 of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system. The recombinant MYT1 fragments containing either the upstream 2 zinc fingers or the downstream 4 zinc fingers bound the same cis regulatory element in the PLP1 promoter in vitro and DNA binding required Zn2+, but not other divalent cations.

Azioni biochim/fisiol

The PKMYT1 (protein kinase, membrane associated tyrosine/threonine 1) gene expression is essential for disease progression of colorectal cancer and hepatocellular carcinoma. The action of Cdc2 (cyclin-dependent kinase 2), an essential molecule required for the induction of mitosis, is regulated by PKMYT1. PKMYT1 binds to the promoter regions of proteolipid proteins of the central nervous system and plays a role in the developing nervous system.

Stato fisico

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Nota sulla preparazione

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable