SRP0730

PIP5K3 (PIKFYVE), Active

PRECISIO^® Kinase, recombinant

• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Ricombinante: expressed in baculovirus infected Sf9 cells
• Saggio: 70-95% (SDS-PAGE)
• Forma fisica: frozen liquid
• Attività specifica: 90-115 nmol/min·mg (ADP-Glo)
• PM: ~110 kDa
• Concentrazione: 0.05-0.1 μg/μL
• Numero d’accesso: NM_015040
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... PIP5K3(200576)

Descrizione

Applicazioni

Useful for kinase assay.

Azioni biochim/fisiol

Recombinant human PIP5K3 (1400-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. PIP5K3 (also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) encodes an enzyme that phosphorylates the D-5 position in PtdIns and phospha-tidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns (3,5)biphosphate. PIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol but also have protein kinase activity ⁽¹⁾. PIP5K3 regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. The inhibition of PIP5K3 renders the late endosome /lysosome compartment refractory to fusion with autop-hagosomes and multivesicular bodies ⁽²⁾.

Stoccaggio e stabilità

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Prodotti consigliati

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Note legali

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable