SRP0236

PI3 kinase (p110d/p85a) Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Sinonimo/i: PI3-kinase p110 subunit delta, PIK3CD, Phosphoinositide-3-kinase, catalytic, delta, ptdIns-3-kinase subunit p110-δ
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in baculovirus infected insect cells
• Saggio: ≥80% (SDS-PAGE)
• Forma fisica: aqueous solution
• Attività specifica: ≥1.1 pmol/min-μg
• PM: 146 kDa (p110δ); 83.5 kDa (p85α)
• Confezionamento: pkg of 20 μg
• Concentrazione: >0.02 mg/mL
• N° accesso NCBI: NM_005026 (p110delta); XM_043865 (p85alpha)
• N° accesso UniProt: O00329
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... PIK3CD(5293)

Descrizione

Descrizione generale

Complex of N-terminal GST tagged recombinant full-length human p110δ (GenBank Accession No. NM_005026), and recombinant full length, human p85α, GenBank Accession No. XM_043865). Coexpressed in a Baculovirus infected Sf9 cell expression system.

Applicazioni

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definizione di unità

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H315,H319,H360D
• Consigli di prudenza: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Classi di pericolo: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Codice della classe di stoccaggio: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe di pericolosità dell'acqua (WGK): WGK 1