SAB4200782

Anti-Tenascin antibody, Mouse monoclonal

clone BC-24, purified from hybridoma cell culture

• Sinonimo/i: Anti-Cytotactin, Anti-GMEM, Anti-GP 150-225, Anti-Glioma-associated-extracellular matrix antigen, Anti-Hexabrachion, Anti-JI, Anti-Myotendinous antigen, Anti-Neuronectin, Anti-TN, Anti-Tenascin-C (TN-C)
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Forma dell’anticorpo: purified from hybridoma cell culture
• Tipo di anticorpo: primary antibodies
• Clone: BC-24, monoclonal
• Forma fisica: buffered aqueous solution
• Reattività contro le specie: human
• Concentrazione: ~1.0 mg/mL
• tecniche: ELISA: suitable; flow cytometry: suitable; immunoblotting: suitable; immunohistochemistry: 5-10 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tonsil sections
• Isotipo: IgG1
• N° accesso UniProt: P24821
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TNC(3371)

Descrizione

Descrizione generale

Monoclonal Anti-Tenascin (mouse IgG1 isotype) is derived from the BC-24 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with human tenascin. Tenascin-C (TN-C) is also known as Hexabrachion, Cytotactin, Neuronectin (NEC1). It is a high molecular mass extracellular matrix glycoprotein. Human tenascin is a disulfide-linked hexamer composed of 3 subunits of 190, 200, and 220 kDa.

Immunogeno

Human tenascin

Applicazioni

Anti-Tenascin antibody has been used:

• in immunoblotting
• in Immunohistochemistry
• in flow cytometry
• in enzyme linked immunosorbent assay(ELISA)
• for blocking tenascin-C proliferating activity

Azioni biochim/fisiol

Tenascin-C (TN-C) functions in cell adhesion, fibroblast migration, and other processes related to tissue remodeling and wound healing. It has been proposed that actively growing, migrating and differentiating epithelial sheets can produce factors such as (Transforming growth factor beta) TGF-β to stimulate tenascin expression. Neo-expression or increased expression of tenascin has been found in the stroma of various tumors and during normal tissue repair. Intracytoplasmic tenascin immunoreactivity has been detected in malignant melanomas and in lung carcinomas, and it serves as a marker of stromal element proliferation in invasive breast carcinomas. High-molecular mass tenascin isoform plays a role in generating a permissive environment for proliferation, invasion, and metastasis of neoplastic epithelial cells. Human tenascin contains an Arg-Gly-Asp (RGD) sequence which may function in cell adhesion and mediates cell attachment through an RGD-dependent integrin receptor.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Altre note

This product is for R&D use only, not for drug, household, or other uses.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable