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Key Documents

SAB4200739

Sigma-Aldrich

Anti-Protein kinase C antibody, Mouse monoclonal

clone MC5, purified from hybridoma cell culture

Sinonimo/i:

Anti-PKC

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.44

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified from hybridoma cell culture

Tipo di anticorpo

primary antibodies

Clone

MC5, monoclonal

Forma fisica

buffered aqueous solution

PM

antigen ~80 kDa

Reattività contro le specie

chicken, human, fish, mouse, bovine, rabbit, rat

Concentrazione

~1.0 mg/mL

tecniche

immunoblotting: 1.25-2.5 μg/mL using human bone marrow neuroblast SH-SY5Y cell extract
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable

Isotipo

IgG2a

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... PRKCA(5578)

Descrizione generale

Anti-Protein kinase C antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the MC5 hybridoma produced by the fusion of X-63 myeloma cells and splenocytes from a BALB/c mouse immunized with purified bovine brain protein kinase C1. Protein kinase C (PKC), is a family of phospholipid- and calcium-activated, diacylglycerol (DAG)-dependent serine/threonine kinases.

Specificità

Monoclonal Anti-Protein kinase C antibody recognizes an epitope located within the amino acid residues 296- 317, at the hinge region, close to or at the trypsin cleavage site of Protein kinase C molecule . The antibody specifically recognizes Protein kinase C from human, rat, bovine, mouse, chicken, rabbit and fish origin.

Applicazioni

Anti-Protein kinase C antibody has been used in:
  • immunoblotting
  • immunohistochemistry
  • immunofluorescence
  • immunoprecipitation

Azioni biochim/fisiol

Protein kinase C (PKC) play a central role in a variety of cell processes, including: intracellular signal transduction, cell proliferation, apoptosis, differentiation, migration and adhesion, tumor promotion, cardiac hypertrophy, angiogenesis, platelet function, inflammation and oncogenesis. Neuronal PKC modulates several functions such as nerve cell excitability, neuronal plasticity and release of neurotransmitters. PKC activity is regulated by two mechanisms- firstly by phosphorylation which regulates the active site and subcellular localization of the enzyme, secondly by secondary messengers which facilitate association of PKC on membrane. The PKC activity is strongly associated with tumor initiation and progression and have been implemented as a target for treatment of cancer progression and diabetic retinopathy.

Stato fisico

Purified bovine brain protein kinase C
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stoccaggio e stabilità

Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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S C Kiley et al.
The Journal of biological chemistry, 266(35), 23761-23768 (1991-12-15)
GH4C1 cells, which express Ca(2+)-dependent alpha- and beta- as well as Ca(2+)-independent gamma-, epsilon- and zeta-protein kinase C (PKC) isozymes, provide a cell culture model for studying isozyme-specific properties and functions. Hormonal activation of PKCs regulates the differentiated functions of
R Garg et al.
Oncogene, 33(45), 5225-5237 (2013-12-18)
Since their discovery in the late 1970s, protein kinase C (PKC) isozymes represent one of the most extensively studied signaling kinases. PKCs signal through multiple pathways and control the expression of genes relevant for cell cycle progression, tumorigenesis and metastatic
Protein kinase C (PKC) isozymes and cancer
Kang J H
New Journal of Science, 2014 (2014)
T Ng et al.
Science (New York, N.Y.), 283(5410), 2085-2089 (1999-03-26)
Spatially resolved fluorescence resonance energy transfer (FRET) measured by fluorescence lifetime imaging microscopy (FLIM), provides a method for tracing the catalytic activity of fluorescently tagged proteins inside live cell cultures and enables determination of the functional state of proteins in
Inhibition of protein kinase C enhances angiogenesis induced by platelet-derived growth factor C in hyperglycemic endothelial cells
Moriya J and Ferrara N
Cardiovascular Diabetology, 14(1), 19-19 (2015)

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