SAB4200715

Anti-β-tubulin antibody, Mouse monoclonal

clone 2-28-33, purified from hybridoma cell culture

• Sinonimo/i: Anti-TUBB1
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Forma dell’anticorpo: purified from hybridoma cell culture
• Tipo di anticorpo: primary antibodies
• Clone: 2-28-33, monoclonal
• Forma fisica: buffered aqueous solution
• PM: ~50 kDa
• Reattività contro le specie: dog, chicken, rat, bovine, monkey, human, mouse
• Concentrazione: ~1.0 mg/mL
• tecniche: immunoblotting: 0.5 μg/mL using mouse embryo fibroblast NIH-3T3 cells extract; immunofluorescence: 1-2 μg/mL using human foreskin fibroblast HS68 cells; immunohistochemistry: suitable
• Isotipo: IgG1
• N° accesso UniProt: P07437
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TUBB(203068) ; mouse ... Tubb3(22152)

Descrizione

Descrizione generale

Anti-β-tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 2-28-33 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin also known as TUBB is the major building block of microtubules. Microtubules assemble from heterodimers of α-tubulin and β-tubulin; both have a molecular weight of ~ 50 kDa and share considerable homology. For β-tubulin, five evolutionarily conserved isotypes have been identified, which are regularly utilized in the same cell types of different species.

Immunogeno

Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Applicazioni

Monoclonal Anti-β-tubulin has been used:

• in immunoblotting
• in immunofluorescence
• in immunohistochemistry

Azioni biochim/fisiol

Monoclonal Anti-β-tubulin specifically recognizes the two major β-tubulin isotypes and one of the minor β-tubulin isotypes of the free-living soil nematode Caenorhabditis elegans. It also binds to Sarkosyl resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, neuronal axons of the larva of Ciona intestinalis (ascidian), microtubules in root tip cells in Arabidopsis thaliana and to human, mouse, rat, monkey, bovine, dog and chicken β-tubulin. The different β-tubulin isotypes are suggested to differ from each other in their ability to polymerize into microtubules.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable