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Key Documents

SAB4200372

Sigma-Aldrich

Anti-WASH1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Sinonimo/i:

Anti-FAM39E, Anti-WAS protein family homolog 1, Anti-WASH

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rabbit

Coniugato

unconjugated

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

PM

antigen ~72 kDa

Reattività contro le specie

human, rat, mouse

Concentrazione

~1.0 mg/mL

tecniche

immunoprecipitation (IP): 5-10 μg using lysates of rat NRK cells.
western blot: 2-4 μg/mL using whole extracts of HEK-293T cells over-expressing mouse WASH1.

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

Descrizione generale

WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog) is a new member of the WASP family. Similar to other WASP family members, it contains a C-terminal for ′WH2, connecting and acidic′ (WCA) domain that binds to Actin related protein 2/actin related protein 3 (Arp2/3). In addition, WASH1 also contains a short proline-rich region, a unique N-terminal domain termed WASH-homology domain (WAHD1), and a tubulin-binding region.

Immunogeno

synthetic peptide corresponding to an internal region of human WASH1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

Applicazioni

Anti-WASH1 antibody produced in rabbit has been used in western blotting and immunoprecipitation.

Azioni biochim/fisiol

WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog), is a nucleation-promoting factor at the surface of endosomes. It recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of endosomes. WASH1 forms part of a multiprotein complex composed of FAM21, KIAA1033 strumpellin and WASH-interacting protein (SWIP) and coiled coil domain containing 53 (CCDC53). It associates with tubulin and localizes to early and recycling endosomes, where together with the Arp2/3 complex and actin, it is required for maintaining the shape of the endosomal compartment and the regulation of the retrograde transport.

Stato fisico

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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DNAJC13 p. Asn855Ser, implicated in familial parkinsonism, alters membrane dynamics of sorting nexin 1
Follett J, et al.
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European journal of human genetics : EJHG, 23(5), 633-638 (2014-06-12)
Ritscher-Schinzel syndrome (RSS)/3C (cranio-cerebro-cardiac) syndrome (OMIM#220210) is a rare and clinically heterogeneous developmental disorder characterized by intellectual disability, cerebellar brain malformations, congenital heart defects, and craniofacial abnormalities. A recent study of a Canadian cohort identified homozygous sequence variants in the
WASH and the Arp2/3 complex regulate endosome shape and trafficking
Duleh SN and Welch MaD
Cytoskeleton (Hoboken, N.J.), 67(3), 193-206 (2010)
A FAM21-containing WASH complex regulates retromer-dependent sorting
Gomez TS and Billadeau DD
Developmental Cell, 17(5), 699-711 (2009)

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