SAB3701031

Anti-Mouse IgG (Fc specific), F(ab′)₂ fragment, highly cross adsorbed-Biotin antibody produced in goat

affinity isolated antibody, lyophilized powder

• Codice UNSPSC: 12352203
• NACRES: NA.46

Proprietà

• Origine biologica: goat
• Coniugato: biotin conjugate
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: secondary antibodies
• Clone: polyclonal
• Forma fisica: lyophilized powder
• Reattività contro le specie: mouse
• tecniche: immunohistochemistry: suitable; indirect ELISA: suitable; western blot: suitable
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. The Fc fragment contains the CH2 and CH3 domains of the antibody molecule. Pepsin cleaves the carboxy-terminal side of the disulfide bonds in the general region of the antibody and this gives rise to the F(ab′)2 fragment. The two antigen-binding arms of the antibody are linked in this fragment. Maternal IgG is the only antibody transported across the placenta to the fetus. It passively immunizes the infants.

Specificità

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Biotin, Anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Anti-Pepsin, Anti-Goat IgG F(c), Mouse IgG F(ab′)2 or Bovine, Horse and Human Serum Proteins.

Immunogeno

Mouse IgG F(c) fragment

Proprietà fisiche

Antibody format: IgG F(ab′)2

Stato fisico

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Ricostituzione

Reconstitute with 500 μ;L deionized water (or equivalent).

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Pittogrammi: GHS06,GHS09
• Avvertenze: Danger
• Indicazioni di pericolo: H302,H311,H411
• Consigli di prudenza: P280 - P301 + P312 + P330 - P302 + P352 + P312
• Classi di pericolo: Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2
• Rischi supp: EUH032
• Codice della classe di stoccaggio: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable