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Key Documents

S1577

Sigma-Aldrich

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor human

recombinant, expressed in E. coli, lyophilized powder, ≥97% (SDS-PAGE), suitable for cell culture

Sinonimo/i:

SDF-1, SDF1, SDF-1α

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.32

Origine biologica

human

Livello qualitativo

Ricombinante

expressed in E. coli

Saggio

≥97% (SDS-PAGE)

Forma fisica

lyophilized powder

Potenza

≤50 ng/mL EC50

Confezionamento

pkg of 10 μg

Condizioni di stoccaggio

avoid repeated freeze/thaw cycles

tecniche

cell culture | mammalian: suitable

Impurezze

≤1 EU/μg protein Endotoxin

Colore

white

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... CXCL12((6387)

Descrizione generale

Stromal Cell-Derived Factor 1α (SDF-1α) or pre-B Cell Growth Stimulation Factor (PBSF) is an 8 kDa chemokine peptide. SDF-1 was identified using signal sequence trap cloning. With this method, cDNAs have been cloned using mouse and human stromal cell lines. This cytokine is widely accepted as a CXC chemokine of the intercrine-macrophage inflammatory protein superfamily. The gene has been mapped to chromosome 10q and is encoded by 4 exons. Expression of the human SDF1 gene is abundant in the pancreas, spleen, ovary, and small intestine. The presence of a GC-rich sequence and the lack of a TATA box in the 5∅-flanking region of the gene are consistent with ubiquitous expression.

Azioni biochim/fisiol

SDF-1α functions as a pre-B cell growth factor in the presence of IL-7 (interleukin-7) and a potent chemoattractant for T-lymphocytes and monocytes. It has also been found to be a ligand for CXCR4 (chemokine receptor type 4) or the orphan receptor LESTR/fusin (putative chemokine receptor). By signaling through the receptor, SDF1 may serve as an inhibitor of HIV-1 (human immunodeficiency virus), which utilizes the LESTR/fusin receptor as a point of entry. The SDF-1/CXCR4 signalling pathway is associated with cancer cell proliferation, migration and survival. Overexpression of SDF1 is observed in breast carcinoma, ovarian carcinoma and papillary thyroid carcinoma. It enhances angiogenesis in the ischemic tissue. SDF1 offers CXCR4 mediated neuroprotection during post ischemic stroke.

Stato fisico

This product is lyophilized from a 0.2 μm-filtered solution containing 10 μg SDF-1α and 0.5 mg bovine serum albumin in PBS.

Ricostituzione

Reconstitute the contents of the vial using sterile buffer or medium containing a minimum of 0.1% BSA or HSA to a stock concentration of ≥10 μg/ml. Additional filtration of the stock solution is not recommended as this may result in loss of product due to adsorption onto the filter membrane.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Diversity and inter-connections in the CXCR4 chemokine receptor/ligand family: molecular perspectives
Pawig L, et al.
Frontiers in Immunology (2015)
Structure and Chromosomal Localization of the Human Stromal Cell-Derived Factor 1 (SDF1) Gene
Shirozu M, et al.
Genomics, 28, 495-500 (1995)
SDF-1/CXCR4 expression is an independent negative prognostic biomarker in patients with head and neck cancer after primary radiochemotherapy
Colle CD, et al.
Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology, 126, 125-131 (2018)
The α-Chemokine, Stromal Cell-derived Factor-1α, Binds to the
Transmembrane G-protein-coupled CXCR-4 Receptor and
Activates Multiple Signal Transduction Pathways.
Ganju RK, et al.
The Journal of Biological Chemistry, 273, 23169?- 233175 (1998)
Wnt/?-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells
International Journal of Clinical and Experimental Pathology, 8(10), 12357?12367-12357?12367 (2015)

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