R8381

Dpn I from Diplococcus pneumoniae

Restriction Enzyme

• Numero CAS: 81295-14-9
• Classificazione EC (Enzyme Commission): 3.1.21.4 (BRENDA, IUBMB)
• Numero MDL: MFCD00166565
• Codice UNSPSC: 12352204

Proprietà

• Grado: for molecular biology
• Forma fisica: buffered aqueous glycerol solution
• Concentrazione: 10,000 units/mL
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Specificità

Recognition sequence: 5′-G^mA/TC-3′
Ligation and recutting results: After 2-10-fold Dpn I overdigestion of 1 μg pBR322 DNA substrate, results in 100% cutting, >30% of fragments can be ligated, and >90% recut.
Heat inactivation: 75 °C for 15 minutes.

Applicazioni

DpnI is a restriction endonuclease that is used in molecular biological applications to cleave the recognition sequence 5′-G^mA/TC-3′, generating DNA framents with blunt ends.

Altre note

Supplied with 10x Restriction Enzyme Buffer SA (B7531).

Comment: Since Dpn I will completely cleave only fully methylated pBR322 DNA, cleavage of 95% or more is considered complete digestion.

Stato fisico

Solution in 10 mM Tris-HCl, pH 8.0, 400 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol (v/v) at 4 °C

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable