R5780

Anti-Rab4 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Anti-HRES-1/RAB4, Anti-RAB4A, Anti-RAS-associated protein RAB4
• Numero MDL: MFCD01634072
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• PM: antigen ~24 kDa
• Reattività contro le specie: human, rat, mouse
• Concentrazione: ~1.0 mg/mL
• tecniche: indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 and rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.1% and 0.4% saponin, respectively.; western blot: 2-5 μg/mL using whole extract of human HEK-293T cells
• N° accesso UniProt: P20338
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... RAB4A(5867) ; mouse ... Rab4a(19341) ; rat ... Rab4a(25532)

Descrizione

Descrizione generale

Rab4 is a GTPase , that is located in early and recycling endosomes. It belongs to Rab family and is located on human chromosome 1q42. In rat testis, Rab4 is found in sertoli and germ cells.

There are two highly homologous RAB4 isoforms, RAB4A and RAB4B, encoded by two separate genes (RAB4A and RAB4B). RAB4A and RAB4B are localized in the same cellular compartments and are believed to have similar or identical functions in recycling.

Applicazioni

The antibody can be used in several immunochemical techniques including immunoblotting (24 kDa) and immunofluorescence.

Azioni biochim/fisiol

Rab4 regulates endocytic recycling in a cell cycle dependent fashion. Rab4 is associated with early endosomes and recycling endosomes and regulates the recycling of membranes and proteins from these compartments back to the plasma membrane. Rab4 interacts with several Rab4 effector proteins like Rabip4, that regulates integrin trafficking and is involved in the migration of NIH 3T3 fibroblasts.

Rab4a controls integrin trafficking throughout cell adhesion. It helps in the quick reprocess of receptors from early endosomes to the cell surface. It also controls the secretion of procathepsin L in human melanoma cells.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)