R4279

Anti-RbAp46, C-terminal antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Anti-RBBP7, Anti-Retinoblastoma-binding protein 7
• Numero MDL: MFCD08705513
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• PM: antigen 46 kDa
• Reattività contro le specie: human
• tecniche: indirect immunofluorescence: 2-4 μg/mL using human HeLa cells fixed with paraformaldehyde-Triton; western blot: 0.5-1 μg/mL using nuclear extracts of human HeLa cells
• N° accesso UniProt: Q16576
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... RBBP7(5931)

Descrizione

Descrizione generale

Retinoblastoma-associated binding protein 46 (RbAp46) is also termed as retinoblastoma-binding protein 7 (RBBP7) and has 89% amino acid identity with RbAp48. It belongs to the WD-repeat protein family and has four repeats that ends with Trp-Asp(WD) residues. This protein is a vital component of the histone modifying and remodeling complexes. RBBP7 is mapped to human chromosome Xp22.13

Specificità

Anti-RbAp46, C-terminal specifically recognizes human RbAp46.

Immunogeno

synthetic peptide corresponding to amino acids 411-425 of human RbAp46, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is conserved in human and differs from the mouse sequence by one amino acid.

Applicazioni

Anti-RbAp46, C-terminal antibody produced in rabbit has been used in western blotting and immunofluorescence.

Azioni biochim/fisiol

Retinoblastoma-associated binding protein 46 (RbAp46) plays an important role in modification and remodeling of chromatin during cell growth and differentiation. It plays a key role in the nucleosomal remodeling and deacetylation complex (NURD). It may also involve in histone deacetylation and transcription repression.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stoccaggio e stabilità

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)