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Key Documents

R128

Sigma-Aldrich

Monoclonal Anti-Ryanodine Receptor antibody produced in mouse

clone C3-33, ascites fluid

Sinonimo/i:

Anti-CCO, Anti-KDS, Anti-MHS, Anti-MHS1, Anti-PPP1R137, Anti-RYDR, Anti-RYR, Anti-RYR-1, Anti-SKRR

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

ascites fluid

Tipo di anticorpo

primary antibodies

Clone

C3-33, monoclonal

PM

antigen ~565 kDa (non-mammalian vertebrates, a doublet at 565 kDa represents the α and β isoforms)

Reattività contro le specie

canine, rabbit, rat, chicken, amphibian, guinea pig, mouse, fish, human

Concentrazione

~1 mg/mL

tecniche

immunohistochemistry (frozen sections): 1 μg/mL using 4% paraformaldehyde-fixed, frozen mouse brain tissue
immunoprecipitation (IP): suitable
western blot (chemiluminescent): 1 μg/mL

Isotipo

IgG1

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

Descrizione generale

RyR1 (ryanodine receptor isoform 1) is a homotetrameric intracellular calcium channel, that is present in skeletal muscle tissue. It is located on human chromosome 19q13.1. In rats, RyR1 and RyR2 are expressed in PASMCs (pulmonary artery smooth muscle cells).

Specificità

The antibody reacts strongly with RyR-2 (expressed predominantly in the heart muscle, but also found in stomach, endothelial cells and diffuse areas of the brain; also known as the β isoform), and weakly with RyR-1 (expressed predominantly in skeletal muscle and areas of the brain; also known as the α isoform). It reacts with ryanodine receptor in cardiac muscle of canine, rat, finch and pigeon; in visceral smooth muscle of toad; and in rat brain. It reacts with skeletal muscle of fish and the β isoform of frog, but only weakly with skeletal muscle in rabbit and the α isoform of frog. In immunohistochemistry, the antibody stains are consistent with the endoplasmic reticulum localization of the receptor in the hippocampus.

Immunogeno

dog cardiac ryanodine receptors (RyR)

Applicazioni

Anti-Ryanodine Receptor has been used in immunofluorescence assay.

Azioni biochim/fisiol

In rats PASMCs (pulmonary artery smooth muscle cells), RyR2 (ryanodine receptor isoform 1) participates in RyR-gated Ca2+ entry. It controls the secretion of insulin and glucose homeostasis. After hemorrhagic shock, RyR2 involves in the progress of vascular bi-phasic reactivity to NE (norepinephrine). In human, RyR1 plays an important role in muscle contraction. RYR1 mutation causes CCD (central core disease) and MHS (malignant hyperthermia susceptibility).

Stato fisico

Containing PBS and 0.05% sodium azide

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Internal structure and visualization of transmembrane domains of the RyR1 calcium release channel by cryo-EM
Samso M, et al.
Nature Structural and Molecular Biology, 12(6), 539-544 (2005)
Imaging Ca2+ nanosparks in heart with a new targeted biosensor
Shang W, et al.
Circulation Research, 114(3), 412-420 (2014)
An autosomal dominant congenital myopathy with cores and rods is associated with a neomutation in the RYR1 gene encoding the skeletal muscle ryanodine receptor
Monnier N, et al.
Human Molecular Genetics, 9(18), 2599-2608 (2000)
The pore structure of the closed RyR1 channel
Ludtke SJ, et al.
Structure, 13(8), 1203-1211 (2005)
Glucose-Dependent Insulin Secretion in Pancreatic ?-Cell Islets from Male Rats Requires Ca2+ Release via ROS-Stimulated Ryanodine Receptors
Llanos P, et al.
PLoS ONE, 10(6) (2015)

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