P9155

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt 30,000-70,000, powder, γ-irradiated, suitable for cell culture, BioXtra

• Sinonimo/i: PDL HBr
• Numero CAS: 25988-63-0
• Numero MDL: MFCD00237230
• Codice UNSPSC: 12352202
• eCl@ss: 32160406
• ID PubChem: 24899014
• NACRES: NA.75

Proprietà

• product name: Poli-L-lisina, mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
• Origine biologica: synthetic (organic)
• Livello qualitativo: 200
• Sterilità: γ-irradiated
• Nome Commerciale: BioXtra
• Forma fisica: lyophilized powder
• PM: 30,000-70,000
• Confezionamento: pkg of 5 mg
• tecniche: cell culture | mammalian: suitable
• Copertura della superficie: 4 μg/cm²
• Solubilità: H₂O: soluble 50 mg/mL, clear, colorless
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C
• Stringa SMILE: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1; WBSCNDJQPKSPII-KKUMJFAQSA-N

Descrizione

Descrizione generale

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties. Poly-L-lysine hydrobromide is a nonspecific attachment factor in promoting cell adhesion to the substratum.

Applicazioni

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm². Optimal conditions for attachment must be determined for each cell line and application.
Poly-L-lysine hydrobromide has been used to adhere the dissociated cells of cortical tissues into the culture plates. It has also been used to treat slides, to fix samples on it for immunohistochemical analysis.

Azioni biochim/fisiol

Poly-L-lysine is employed as a coating substance to facilitate improved cell attachment to culture surfaces. Research indicates that plates coated with poly-L-lysine (PLL) exhibit an accelerated cell growth rate. When mesenchymal stem cells (MSCs) are cultured on PLL-coated substrates, it helps preserve their stemness properties and prevents senescence. PLL-coated plates promote enhanced MSC growth and up-regulation of genes associated with cell adhesion, differentiation, proliferation, and signaling. Furthermore, porous PLL-coated surfaces have been shown to enhance cell proliferation and support the osteogenic differentiation of MSCs.

Componenti

La poli-L-lisina è un polimero aminoacidico con carica positiva con circa un HBr per residuo di lisina. Il bromidrato consente alla poli-L-lisina di raggiungere la forma cristallina solubile in acqua. Una piccola quantità del prodotto può essere rinvenuta nella struttura beta perché il HBr interferisce con il legame idrogeno tra i gruppi amminici e i gruppi carbossilici o le frazioni che contengono N o O.

Avvertenza

Le soluzioni sterili sono stabili per un massimo di due anni se conservate a 2-8 °C. Il prodotto deve essere conservato essiccato a -20 °C.

Risultati analitici

Molecular weight based on viscosity. Also assayed by MALLS.

Altre note

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)