P8045
Protein G-Biotin from Streptococcus sp.
recombinant, expressed in unspecified host, lyophilized powder
Sinonimo/i:
Biotinylated Protein G, Protein G Biotin, Protein G-Biotin
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About This Item
Prodotti consigliati
Ricombinante
expressed in unspecified host
Livello qualitativo
Coniugato
biotin conjugate
Forma fisica
lyophilized powder
Composizione
Protein, ~90% biuret
Grado di funzionalizzazione
2-4 mol d-biotin per mol protein
tecniche
direct ELISA: 1:70,000 using human IgG
Temperatura di conservazione
2-8°C
Descrizione generale
Protein G binds to antibodies reversibly and thus, it is considered in antibody purification. It is a 20 kDa protein.
Protein G is an immunoglobulin (IgG) specific bacterial cell wall protein isolated from Streptococci (group G). Native proteins G consist of two IgG-binding domains and albumin and cell surface binding sites. Biotin is a water soluble vitamin that acts as a coenzyme for carboxylase enzyme reactions. Biotin deficiency results in deregulation of metabolic pathways and various physiological impairments. Biotin deficiency is known to cause birth anomalies indicating its important role in development and differentiation of embryos.
Specificità
Binds IgG only from most mammalian species except cat; binds chicken IgG.
Applicazioni
Protein G-Biotin from Streptococcus sp. has been used during incubation for synergistic immunocapture of antigen which was performed using streptavidin-coated magnetic beads.
Protein G-Biotin from Streptococcus sp. has been used in electrochemical biosensing, for the attachment of antibodies with different specificities.
Confezionamento
Package size based on protein content
Altre note
Truncated - lacks albumin, Fab and membrane binding sites.
Nota sulla preparazione
Prepared using biotinamidocaproate N-hydroxysuccinimide which incorporates an aminocaproyl spacer.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
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