P354-05
Porcine Aortic Smooth Muscle Cells: PAOSMC (Cryovial)
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About This Item
Codice UNSPSC:
41106514
NACRES:
NA.81
Prodotti consigliati
Origine biologica
Porcine aorta (normal)
Livello qualitativo
Forma fisica
solid
Confezionamento
pkg of 500,000 cells
Produttore/marchio commerciale
Cell Applications, Inc
Modalità di accrescimento
Adherent
Cariotipo
2n = 38
Morfologia
smooth muscle
tecniche
cell culture | mammalian: suitable
Malattie correlate
cardiovascular diseases
Condizioni di spedizione
dry ice
Temperatura di conservazione
−196°C
Descrizione generale
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PAOSMC were used to investigate the effects of heparin on smooth muscle cell proliferation, specifically revealing that heparin induces MKP-1 phosphatase that dephosphorylates ERK, decreasing its activity (Blaukovitch, 2010). They were also used to show that normalization of myocardin signaling in the diseased heart by using targeted inhibition of myocardin genes by shRNA restores heart function (Torrado, 2011). Finally, PAOSMC along with Porcine Aortic Endothelial cells, have been used as controls in a study characterizing circulating endothelial progenitor cells (Allen, 2008).
Characterization: positive for smooth muscle cell specific alpha-actin expression.
PAOSMC were used to investigate the effects of heparin on smooth muscle cell proliferation, specifically revealing that heparin induces MKP-1 phosphatase that dephosphorylates ERK, decreasing its activity (Blaukovitch, 2010). They were also used to show that normalization of myocardin signaling in the diseased heart by using targeted inhibition of myocardin genes by shRNA restores heart function (Torrado, 2011). Finally, PAOSMC along with Porcine Aortic Endothelial cells, have been used as controls in a study characterizing circulating endothelial progenitor cells (Allen, 2008).
Characterization: positive for smooth muscle cell specific alpha-actin expression.
Origine della linea cellulare
Aorta
Applicazioni
aortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulation
Componenti
Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
Nota sulla preparazione
- 1st passage, >500,000 cells in Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
- Can be cultured at least 16 doublings
Mantenimento delle subcolture
Please refer to the PAOSMC Culture Protocol.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Nina Parmar et al.
Tissue engineering. Part C, Methods, 21(4), 404-412 (2014-09-11)
Muscle degeneration is a prevalent disease, particularly in aging societies where it has a huge impact on quality of life and incurs colossal health costs. Suitable donor sources of smooth muscle cells are limited and minimally invasive therapeutic approaches are
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
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