P0270

Calmodulin from bovine heart

lyophilized powder, 2,500-10,000 units/mg protein

• Sinonimo/i: Phosphodiesterase 3′:5′-cyclic nucleotide activator from bovine heart
• Numero CAS: 77107-46-1
• Numero MDL: MFCD00131869
• Codice UNSPSC: 12352202
• NACRES: NA.61

Proprietà

• Origine biologica: bovine heart
• Livello qualitativo: 200
• Forma fisica: lyophilized powder
• Attività specifica: 2,500-10,000 units/mg protein
• Composizione: Protein, ≥85% Lowry
• tecniche: calcium flux assay: suitable
• N° accesso UniProt: P62157
• Temperatura di conservazione: −20°C
• Informazioni sul gene: cow ... CALM3(520277)

Descrizione

Applicazioni

This is the protein activator required for the Ca²⁺ dependent activation of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520.

Azioni biochim/fisiol

Ca²⁺ binding protein that is required for activation of cyclic nucleotide-dependent phosphodiesterase. It is also a cofactor/activator of nitric oxide synthase, calcineurin, and many kinases including ATPase, myosin light chain kinase, and CAM kinase I, II, and III. It mediates ryanodine receptor activation by cyclic ADP ribose and is involved in intracellular Ca²⁺ homeostasis.

Definizione di unità

One unit will stimulate 0.008 activated unit of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520, in a 3 mL reaction volume at pH 7.5 and 30 °C, to 50% of the maximum activity of the enzyme when saturated with activator, in the presence of 0.01 mM Ca²⁺.

Stato fisico

Lyophilized powder containing buffer salts as imidazole and magnesium sulfate

Nota sulla preparazione

Purified by a modification of the procedure of Teo, T.S., et al., J. Biol. Chem., 248, 588 (1973).

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)