N9287

Monoclonal Anti-NBS1 (Nibrin) antibody produced in mouse

~2 mg/mL, clone NBS1-501, purified immunoglobulin, buffered aqueous solution

• Sinonimo/i: Anti-Nijmegen Breakage Syndrome, Anti-p95 Protein of NBS1.MRE11/RAD50 Complex
• Numero MDL: MFCD03455620
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Coniugato: unconjugated
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: NBS1-501, monoclonal
• Forma fisica: buffered aqueous solution
• PM: antigen ~95 kDa
• Reattività contro le specie: human
• Concentrazione: ~2 mg/mL
• tecniche: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 2-4 μg/mL using nuclear extract of HEK 293T expressing recombinant human NBS1
• Isotipo: IgG1
• N° accesso UniProt: O60934
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... NBN(4683)

Descrizione

Descrizione generale

Monoclonal Anti-NBS1 (Nibrin) (mouse IgG1isotype) is derived from the hybridoma NBS1-501 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human NBS1. Nibrin is mapped to human chromosome 8q21.3. The encoded protein has two domains found in the cell cycle checkpoint proteins, forkhead-associated domain (FHA), and an adjacent breast cancer carboxy-terminal domain (BRCT).

Immunogeno

synthetic peptide corresponding to amino acids 206-220 of human NBS1.

Applicazioni

Monoclonal Anti-NBS1 (Nibrin) antibody produced in mouse has been used in:

• antibody microarray
• immunocytochemistry
• immunoblotting
• immunoprecipitation
• enzyme linked immunosorbent assay (ELISA)

Azioni biochim/fisiol

NBS1(Nibrin) was first isolated as a protein involved in DNA repair through analysis of mutations in patients with this syndrome. p95/NBS1(Nibrin) deficiency abrogates the formation of the Meiotic recombination 11 homolog 1(MRE11) / DNA repair protein RAD50 ionizing radiation-induced foci, revealing a molecular link between double-stranded break repair (DSB) and cell cycle checkpoint functions. The phenotypic similarities between ataxia-telangiectasia (AT) and Nijmegen breakage syndrome had suggested that ataxia-telangiectasia mutated gene (ATM) and NBS1 functions in a common signaling pathway. This was confirmed by the finding that in response to ionizing radiation, NBS1is phosphorylated in Ser343 in an ataxia-telangiectasia mutated ATM-dependent manner.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable