N6514

Neuraminidase from Vibrio cholerae

Type II, buffered aqueous solution, 8-24 units/mg protein (Lowry, using NAN-lactose)

• Sinonimo/i: Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase
• Numero CAS: 9001-67-6
• Classificazione EC (Enzyme Commission): 3.2.1.18 (BRENDA, IUBMB)
• Numero CE: 232-624-6
• Numero MDL: MFCD00131711
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Origine biologica: Vibrio cholerae
• Livello qualitativo: 200
• Tipo: Type II
• Forma fisica: buffered aqueous solution
• Attività specifica: 8-24 units/mg protein (Lowry, using NAN-lactose)
• Attività estranea: Protease and NAN-aldolase, present
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Calorimetry studies show that the two lectin-like domains flanking the central catalytic domain of Neuraminidase serve as the recognition and binding sites for sialic acid-containing substrates.

Neuraminidase enzymes are hydrolase enzymes that promote influenza virus release from infected cells and facilitate virus spread.

Applicazioni

Neuraminidase from Vibrio cholera has been used in a study to describe a five-step purification method. It has also been used in a study to investigate modification of leukemia L1210 tumor cells.

Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.

Qualità

Preservative free.

Definizione di unità

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

Stato fisico

Solution in 50 mM sodium acetate, pH 5.5, containing 0.15 M sodium chloride and 4 mM calcium chloride, 0.2 μm filtered..

Nota sulla preparazione

A further purification by affinity chromatography of our Type III.

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P284 - P501
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)