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Key Documents

N2787

Sigma-Aldrich

Monoclonal Anti-Neurofilament 160 antibody produced in mouse

~2 mg/mL, clone RMO44, purified immunoglobulin, buffered aqueous solution

Sinonimo/i:

Anti-Neurofilament Medium Chain

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Coniugato

unconjugated

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

RMO44, monoclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

human, mouse, rat, zebrafish

Concentrazione

~2 mg/mL

tecniche

immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using extract of SHSy5y human neuroblastoma cells

Isotipo

IgG1

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... NEFM(4741)
mouse ... Nefm(18040)
rat ... Nefm(24588)

Descrizione generale

Monoclonal Anti- Neurofilament 160 (mouse IgG1 isotype) is derived from the hybridoma RMO44 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified mid-sized rat neurofilament (NF-M) subunit. Neurofilaments are one of the five major groups of intermediate filaments (Ifs) and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kDa, 160 kDa and 200 kDa.
Neurofilament 160 (NEFM) is one among the most abundant filaments in post-mitotic neurons.

Immunogeno

purified mid-sized rat neurofilament (NF-M) subunit.

Applicazioni

Monoclonal Anti-Neurofilament 160 antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • western blot analysis
  • immunohistochemistry
  • antibody labelling

Azioni biochim/fisiol

Neurofilament 160 (NEFM) associates with the dopamine receptor.
Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals. These molecules undergo post-translational modifications, which results in their heterogeneity including different levels of phosphorylation. The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing their interaction with cytoplasmic organelles.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Raccomandato

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Neurofilaments at a glance
Yuan A, et al.
Journal of Cell Science, 125(14), 3257-3263 (2012)
An in vitro model for studying CNS white matter: functional properties and experimental approaches
Bijland S, et al.
F1000Research, 8(1) (2019)
Distribution patterns of the zebrafish neuronal intermediate filaments inaa and inab
Liao ML, et al.
Journal of Neuroscience Research, 97(2), 202-214 (2019)
Neuronal cytoskeletal alterations in an experimental model of depression
Reinees A, et al.
Neuroscience, 3-3 (2004)
The amount of neurofilaments aggregated in the cell body is controlled by their increased sensitivity to trypsin-like proteases
Fasani F, et al.
Journal of Cell Science, 117(6), 861-869 (2004)

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