MTOX1011

PXR Knockout HepaRG^™ Cells

human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)

• Codice UNSPSC: 12352207
• NACRES: NA.81

Proprietà

• product name: PXR Knockout HepaRG^™ Cells, 1 vial
• Origine biologica: human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)
• Forma fisica: liquid
• N° accesso OMIM: 603065
• Temperatura di conservazione: −196°C
• Informazioni sul gene: human ... NR1I2(1337040)

Descrizione

Descrizione generale

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG PXR KO cells express the major xenobiotic sensors (CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

Applicazioni

See technical bulletin for detailed protocols

Caratteristiche e vantaggi

HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity.

• Sigma′s HepaRG PXR Knockout (KO) allows conclusive identification of CYP3A4 induction through PXR activation.

Zinc finger nucleases (ZFN) mediated Knock-out of NR1I2 (PXR) gene.

• The frame-shift mutation of NR1I2 gene was confirmed by fragment length analysis and DNA sequencing.
• Loss of functionality was confirmed by loss of response to inducers of cytochrome P450 activity.

Qualità

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

Note legali

These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.

HepaRG is a trademark of BioPredic International company

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable