MAK084
Glucose Uptake Fluorometric Assay Kit
sufficient for 100 fluorometric tests
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About This Item
Codice UNSPSC:
12161503
NACRES:
NA.84
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impiego
sufficient for 100 fluorometric tests
Metodo di rivelazione
fluorometric
Malattie correlate
endocrinological disorders, diabetes; cancer
Temperatura di conservazione
−20°C
Descrizione generale
The new Glucose Uptake Assay Kit, MAK489, is now available! Glucose is the primary source of energy for most cells.Transport of glucose across the plasma membrane is the first rate limiting step in glucose metabolism. Glucose uptake is facilitated by the GLUT family of transporter proteins, whose expression and activity are regulated by multiple mechanisms. Glucose uptake is upregulated in many cancer cells, which exhibit high rates of aerobic glycolysis. Cells exhibiting insulin resistance show diminished glucose uptake in response to insulin stimulation.
Applicazioni
Glucose Uptake Fluorometric Assay Kit has been used to measure the amount of glucose uptake by monocytic myeloid-derived suppressor cells (M-MDSCs).
Compatibilità
Suitable for detecting glucose uptake in adherent or suspension cells cultured in a 96-well microtiter plate.
Principio
The Glucose Uptake Fluorometric Assay kit provides a simple and direct procedure for measuring glucose uptake in a variety of cells. Glucose uptake is measured using the glucose analog, 2-deoxyglucose (2-DG), which is taken up by cells and phosphorylated by hexokinase to 2-DG6P. 2-DG6P cannot be further metabolized and accumulates in cells, directly proportional to the glucose uptake by cells. In this assay, 2-DG uptake is determined by a coupled enzymatic assay in which the 2-DG6P is oxidized, resulting in the generation of NADPH, which reacts with the probe to generate a fluorometric (λex = 535/λem = 587 nm) product, proportional to the 2-DG taken up by the cell.
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Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 3
Codice della classe di stoccaggio
10 - Combustible liquids
Punto d’infiammabilità (°F)
188.6 °F - closed cup
Punto d’infiammabilità (°C)
87 °C - closed cup
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Tingting Wu et al.
Scientific reports, 6, 20250-20250 (2016-02-03)
CD11b(+) Gr1(+) myeloid-derived suppressor cells (MDSCs) play critical roles in controlling the processes of tumors, infections, autoimmunity and graft rejection. Immunosuppressive drug rapamycin (RPM), targeting on the key cellular metabolism molecule mTOR, is currently used in clinics to treat patients
Mechanisms for insulin resistance: common threads and missing links.
Samuel V T and Gerald I S
Cell, 148(5), 852-871 (2012)
Chapter Twenty-Two Mechanisms and Methods in Glucose Metabolism and Cell Death.
Zhao Y, et al.
Methods in Enzymology, 442, 439-457 (2008)
Yang Zhao et al.
Blood, 131(14), 1587-1599 (2018-02-22)
Monocytes and macrophages play a key role in defending pathogens, removing the dead cells or cell debris, and wound healing. The mammalian target of rapamycin (mTOR) inhibitor rapamycin (RPM) is widely used in clinics to treat patients with organ transplantation
Norio Yamamoto et al.
Analytical biochemistry, 375(2), 397-399 (2008-02-12)
To determine 2-deoxy-D-glucose (2DG) and 2-deoxy-D-glucose 6-phosphate (DG6P) in mouse tissue after injection of 2DG, we have developed a novel assay. This assay is a simple procedure involving incubation of samples with four independent, single reaction mixtures followed by measurement
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