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MAK072

Sigma-Aldrich

Pyruvate Kinase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 100 colorimetric or fluorometric tests

Metodo di rivelazione

colorimetric
fluorometric

Malattie correlate

hematological disorder; cancer

Temperatura di conservazione

−20°C

Descrizione generale

Pyruvate kinase (PK) is an enzyme that catalyzes the final step of glycolysis, the transfer of a phosphate group from phospho(enol)pyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Pyruvate kinase deficiency, due to defects in pyruvate kinase expression or activity, is the second most common cause of hemolytic anemia.

The Pyruvate Kinase Activity Assay Kit provides a simple and direct procedure for measuring pyruvate kinase activity in a variety of biological samples. Pyruvate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate present. One unit of pyruvate kinase is the amount of enzyme that will transfer a phosphate group from PEP to ADP to generate 1.0 μmole of pyruvate per minute at 25 °C.

Applicazioni

Pyruvate Kinase Activity Assay Kit has been used to determine the activity of pyruvate kinase in samples.

Compatibilità

Suitable for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc

Principio

The Pyruvate Kinase Activity Assay Kit provides a simple and direct procedure for measuring pyruvate kinase activity in a variety of biological samples. Pyruvate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate present. One unit of pyruvate kinase is the amount of enzyme that will transfer a phosphate group from PEP to ADP to generate 1.0 μmole of pyruvate per minute at 25 °C.

Sostituito da

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H317,H334,H412

Classi di pericolo

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Certificati d'analisi (COA)

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Pyruvate Assay Kit sufficient for 100 colorimetric or fluorometric tests

Sigma-Aldrich

MAK332

Pyruvate Assay Kit

Pyruvate Kinase from rabbit muscle Type II, ammonium sulfate suspension, 350-600 units/mg protein

Sigma-Aldrich

P1506

Pyruvate Kinase from rabbit muscle

PKM1 active human recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Sigma-Aldrich

SRP0415

PKM1 active human

Over expression of hyaluronan promotes progression of HCC via CD44-mediated pyruvate kinase M2 nuclear translocation.
L Jing-Huan, et al.
American Journal of Cancer Research, 6(2), 509-509 (2016)
Therapeutic and Biochemical Effects of Garlic (Allium sativum) on Acrylamide Toxicity in Rabbits: Glycolytic Pathway.
Elmahdi B, et al.
Int. J. Pharmacol., 12(4), 429-434 (2016)
Aoi Wakabayashi et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(16), 4434-4439 (2016-04-05)
Whole-exome sequencing has been incredibly successful in identifying causal genetic variants and has revealed a number of novel genes associated with blood and other diseases. One limitation of this approach is that it overlooks mutations in noncoding regulatory elements. Furthermore
HIF1α modulates cell fate reprogramming through early glycolytic shift and upregulation of PDK1--3 and PKM2.
Prigione A, et al.
Stem Cells, 32(2), 364-376 (2014)
Jianshuang Guo et al.
Frontiers in oncology, 9, 993-993 (2019-10-22)
Glioblastoma (GBM) is the most prevalent malignant tumor in the central nervous system. Aerobic glycolysis, featured with elevated glucose consumption and lactate production, confers selective advantages on GBM by utilizing nutrients to support rapid cell proliferation and tumor growth. Pyruvate
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Articoli

Aerobic Glycolysis and the Warburg Effect

We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.

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