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Key Documents

M8177

Sigma-Aldrich

Anti-p38 MAP Kinase, Activated (Diphosphorylated p38) antibody, Mouse monoclonal

clone P38-TY, purified from hybridoma cell culture, buffered aqueous solution

Sinonimo/i:

Anti-MAP Kinase, p38, Activated, Monoclonal Anti-p38 MAP Kinase, Activated (Diphosphorylated p38) antibody produced in mouse

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.44

Origine biologica

mouse

Coniugato

unconjugated

Forma dell’anticorpo

purified from hybridoma cell culture

Tipo di anticorpo

primary antibodies

Clone

P38-TY, monoclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

rat, human, mouse

Concentrazione

~2 mg/mL

tecniche

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1 μg/mL using whole cell extract from a rat fibroblast cell line, Rat1, activated with sorbitol

Isotipo

IgG2a

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... MAPK14(1432)
mouse ... Mapk14(26416)
rat ... Mapk14(81649)

Descrizione generale

Monoclonal Anti-p38 MAP Kinase, Activated (Diphosphorylated p38) (mouse IgG2a isotype) is derived from the P38-TY hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The p38 mitogen-activated protein kinases (MAPK) comprises the stress-activated protein kinase (SAPK) 2b, SAPK3 and SAPK4 (also termed p38β-δ). All these kinases contain a glycine residue in their TXY(Thr−Xaa−Tyr motif) activation motif.

Specificità

Reacts specifically with the active diphosphorylated form of p38 MAP kinase and its closely related isoforms. Does not recognize the non-phosphorylated and mono­phosphory­lated forms of the p38 MAP kinase molecule, or the non-phosphorylated, monophosphorylated and diphosphorylated forms of JNK and ERK-MAP kinases. The epitope recognized by the antibody contains the phosphorylated threonine and tyrosine residues within the regulatory site of active p38 MAP kinase (Thr180-Gly181-Tyr182).

Immunogeno

Synthetic peptide HTDDEMpTGpYVATR corresponding to the phosphorylated form of p38 MAP kinase activation loop, coupled to KLH.

Applicazioni

Monoclonal Anti-p38 MAP Kinase, Activated (Diphosphorylated p38) antibody produced in mouse may be used in immunoblotting, enzyme linked immunosorbent assay (ELISA), immunostaining, western blotting, immunohistochemistry and in immunocytochemistry.

Azioni biochim/fisiol

The p38 mitogen-activated protein kinases (MAPK) are activated by phosphorylation of both tyrosine (Y) and threonine (T) residues organized in a TXY motif. The residue in between the two phosphorylated residues determines the specificity of activation of the MAPKs, and is glutamic acid for extracellular-signal regulated kinase (ERK) (TEY), proline for c-Jun N-terminal kinases (JNK) and glycine for p38 MAPK. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. ERK generally transmits signals leading to cell proliferation, p38 MAPK and JNK are both mostly stress-responsive kinases3 and have been implicated in cell death in several cellular systems. Antibodies reacting specifically with activated p38 MAPK are useful tools in the study of the intracellular location of p38 MAPK enzymes, and in sorting out the signal transduction pathways of the MAPK superfamily.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Nota sulla preparazione

From a culture supernatant of bioreactor grown hybridoma.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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p38 MAP kinases in the heart
Yokota T and Wang Y
Gene, 575(2), 369-376 (2016)
The p38 MAPK stress pathway as a tumor suppressor or more
Mathew L andGuan C
Frontiers in Bioscience, 13, 3581-3581 (2008)
p38 MAP-kinases pathway regulation, function and role in human diseases
Cuenda A and Rousseau S
Biochimica et Biophysica Acta, 1773(8), 1358-1375 (2007)
Paul T G Elkington et al.
Journal of immunology (Baltimore, Md. : 1950), 175(8), 5333-5340 (2005-10-08)
Pulmonary cavitation is vital to the persistence and spread of Mycobacterium tuberculosis (MTb), but mechanisms underlying this lung destruction are poorly understood. Fibrillar type I collagen provides the lung's tensile strength, and only matrix metalloproteinases (MMPs) can degrade it at
Matrix metalloproteinase-1 is regulated in tuberculosis by a p38 MAPK-dependent, p-aminosalicylic acid-sensitive signaling cascade
Rand L, et al.
Journal of Immunology, 182(9), 5865-5872 (2009)

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