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Key Documents

M6313

Sigma-Aldrich

Mimetic Green 1 Ligand Affinity Adsorbent

suspension

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About This Item

Numero MDL:
Codice UNSPSC:
23151817
NACRES:
NA.56

Forma fisica

suspension

Livello qualitativo

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Applicazioni

Ligand affinity adsorbents are used in protein chromatography, affinity chromatography and dye resins. Ligand affinity adsorbents have been used to describe a simple protocol for the purification of Taq Pol from E. coli lysates as well as for the purification of other DNA polymerases.

Pittogrammi

Flame

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Flam. Liq. 3

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

95.0 °F - closed cup

Punto d’infiammabilità (°C)

35 °C - closed cup

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


Certificati d'analisi (COA)

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B V Thage et al.
Journal of applied microbiology, 96(3), 593-602 (2004-02-14)
Purification and characterization of an aminotransferase (AT) specific for the degradation of branched-chain amino acids from Lactobacillus paracasei subsp. paracasei CHCC 2115. The purification protocol consisted of anion exchange chromatography, affinity chromatography and hydrophobic interaction chromatography. The enzyme was found
Michael Kotik et al.
Biochimica et biophysica acta, 1760(2), 245-252 (2005-12-14)
Purification of a novel enantioselective epoxide hydrolase from Aspergillus niger M200 has been achieved using ammonium sulphate precipitation, ionic exchange, hydrophobic interaction, and size-exclusion chromatography, in conjunction with two additional chromatographic steps employing hydroxylapatite, and Mimetic Green. The enzyme was
S C Woodcock et al.
The Biochemical journal, 313 ( Pt 2), 415-421 (1996-01-15)
CysG, also known as uroporphyrinogen III methylase and sirohaem synthase (CysG; EC 2.1.1.107), is a multifunctional enzyme that is able to transform uroporphyrinogen III into sirohaem via two S-adenosyl-L-methionine (AdoMet)-dependent transmethylations, an NAD(+)-dependent dehydrogenation and a ferrochelation. The apparent tight
J Ni et al.
The Journal of biological chemistry, 272(16), 10853-10858 (1997-04-18)
A new member of the human cystatin superfamily, called cystatin E, has been found by expressed sequence tag (EST) sequencing in amniotic cell and fetal skin epithelial cell cDNA libraries. The sequence of a full-length amniotic cell cDNA clone contained
Sotirios Melissis et al.
Biotechnology journal, 2(1), 121-132 (2006-12-22)
The thermostable Thermus aquaticus DNA polymerase (Taq Pol) has been the key factor in transforming the initial PCR method into one with huge impact in molecular biology and biotechnology. Therefore, the development of effective affinity adsorbents for the purification of

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