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L9636

Sigma-Aldrich

D-Lactic Dehydrogenase from Staphylococcus epidermidis

lyophilized powder, ≥80 units/mg solid

Sinonimo/i:

Lactate, (R)-Lactate:NAD+ oxidoreductase, D-LDH

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About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

bacterial (Staphylococcus epidermidis)

Livello qualitativo

Forma fisica

lyophilized powder

Attività specifica

≥80 units/mg solid

Temperatura di conservazione

−20°C

Applicazioni

D-Lactic Dehydrogenase can be used to generate inhibitors of angiotensin converting enzyme by catalyzing the production of the intermediate (R)-2-Hydroxy-4-phenylbutyric acid.
D-Lactic dehydrogenase has been used in a study to assess mechanisms of active transport in isolated membrane vesicles. It has also been used in a study to investigate β-galactoside transport in bacterial membrane preparations.

Definizione di unità

One unit will reduce 1.0 μmole of pyruvate to D-lactate per min at pH 7.0 at 25 °C.

Stato fisico

Lyophilized powder containing primarily dextran

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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D H Kleinschmit et al.
Journal of dairy science, 88(6), 2130-2139 (2005-05-21)
In 2 consecutive years, whole plant corn was ensiled in laboratory silos to investigate the effects of various silage additives on fermentation, dry matter (DM) recovery and aerobic stability. In yr 1, chopped forage was treated with 1) no additive
E M Barnes et al.
Proceedings of the National Academy of Sciences of the United States of America, 66(4), 1190-1198 (1970-08-01)
The transport of beta-galactosides by isolated membrane preparations from Escherichia coli strains containing a functional y gene is markedly stimulated by the conversion of D-lactate to pyruvate. The addition of D-lactate to these membrane preparations produces a 19-fold increase in
Mechanisms of active transport in isolated membrane vesicles. II. The mechanism of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in membrane preparations from Escherichia coli.
H R Kaback et al.
The Journal of biological chemistry, 246(17), 5523-5531 (1971-09-10)
E Schmidt et al.
Journal of biotechnology, 24(3), 315-327 (1992-07-01)
(R)-2-Hydroxy-4-phenylbutyric acid, an intermediate in the manufacture of inhibitors of angiotensin converting enzyme, can be produced continuously in an enzyme membrane reactor by enzymatic reduction of its corresponding alpha-keto acid. D-Lactate dehydrogenase (D-LDH) from Staphylococcus epidermidis was chosen as the
C C Taylor et al.
Journal of dairy science, 85(7), 1793-1800 (2002-08-31)
Chopped barley forage was ensiled untreated or treated with several doses (1 x 10(5) to 1 x 10(6) cfu/g of fresh forage) of Lactobacillus buchneri 40788 in laboratory silos and untreated or treated (4 x 10(5) cfu/g) in a farm

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