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L9420

Sigma-Aldrich

Luciferase from Photinus pyralis (firefly)

recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein

Sinonimo/i:

Luciferase firefly

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About This Item

Numero CAS:
61970-00-1
Classificazione EC (Enzyme Commission):
1.13.12.7 (BRENDA, IUBMB)
Numero MDL:
MFCD00131546
Codice UNSPSC:
12352204
NACRES:
NA.54

Ricombinante

expressed in E. coli

Livello qualitativo

200

Saggio

≥98% (SDS-PAGE)

Forma fisica

buffered aqueous solution

Attività specifica

≥10×1010 units/mg protein

PM

62 kDa

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

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Descrizione generale

Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP.

Applicazioni

Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway.

Definizione di unità

One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.

Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.

Stato fisico

Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol

Pittogrammi

Health hazardExclamation mark
GHS08,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H302,H334,H373

Classi di pericolo

Acute Tox. 4 Oral - Resp. Sens. 1 - STOT RE 2 Oral

Organi bersaglio

Kidney

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

231.8 °F

Punto d’infiammabilità (°C)

111 °C

Certificati d'analisi (COA)

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Griffiths A J F, et al.
An Introduction to Genetic Analysis. (2000)
Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes.
Conti E, et al.
Structure, 4(3), 287-298 (1996)
Reporter gene-facilitated detection of compounds in Arabidopsis leaf extracts that activate the Karrikin signaling pathway.
Sun Y K, et al.
Frontiers in Plant Science, 7, 1799-1799 (2016)
Greta Forlani et al.
Methods in molecular biology (Clifton, N.J.), 1087, 299-313 (2013-10-26)
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Methods in molecular biology (Clifton, N.J.), 1098, 21-32 (2013-10-30)
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