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Documenti fondamentali

GE29-0181-82

HiPrep™ Q High Performance 16/10

Cytiva 29-0181-82, pack of 1 column

Sinonimo/i:

HiPrep Q Column, High Performance Q Column

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About This Item

Codice UNSPSC:
13111000
NACRES:
NA.56

ligand

quaternary amine

Descrizione

Ion Exchanger Type (value)

Confezionamento

pack of 1 column

Produttore/marchio commerciale

Cytiva 29-0181-82

Parametri

1-5 ml/min flow rate
44 psi

Dimensioni del letto

16 mm × 100 mm

Diam. int. colonna

16 mm

Matrice

6% cross-linked agarose

Dimensione particelle

24-44 μm

Dimens. media delle particelle

34 μm

cleaning

1-14

Intervallo di esercizio

2-12

Compatibilità

suitable for bioprocess medium

Descrizione generale

HiPrep Q HP is a 20 mL strong anion exchange chromatography column for high resolution small-scale protein purification. The Q functional group is a quaternary amino group coupled to the matrix via chemically stable ether linkages. Q Sepharose High Performance is a strong anion exchanger charged with high loading capacity over broad pH ranges.

Caratteristiche e vantaggi

  • Packed with Q Sepharose High Performance strong quarternary ammonium anion exchange resin.
  • Small (34 µm) bead size delivers high-performance, high-resolution purifications.
  • 20 mL HiPrep column format compatible with single-pump configurations and chromatography systems such as ÄKTA systems.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

HiPrep is a trademark of Cytiva

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Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

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Lot/Batch Number

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Articoli

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocolli

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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