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Documenti fondamentali

GE28-9782-45

HiScreen, DEAE Fast Flow

Cytiva 28-9782-45

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About This Item

Codice UNSPSC:
47101511
NACRES:
NA.56

ligand

diethylaminoethyl

Descrizione

Ion Exchanger Type (value)

Stato

prepacked column

Crosslinking

cross-linked

Confezionamento

pkg of 1 ea

Produttore/marchio commerciale

Cytiva 28-9782-45

Condizioni di stoccaggio

(20% Ethanol)

Parametri

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

tecniche

liquid chromatography (LC): suitable

Dimensioni del letto

7.7 mm × 100 mm

Volume del letto

4.7 mL

Diam. int. colonna

7.7 mm

Matrice

6% cross-linked agarose

Gruppo funzionale matrice

N+(C2H5)2H functional group

Dimensione particelle

45-165 μm

Diametro medio

90 μm

cleaning

2-14

Intervallo di esercizio

2-12

Compatibilità

suitable for bioprocess medium

Tecnica di separazione

anion exchange

Descrizione generale

HiScreen DEAE FF columns are prepacked with DEAE Sepharose Fast Flow, a weak anion exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

Applicazioni

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.

If necessary, two columns can easily be connected in series to give a bed height of 20 cm.

Media prepacked in HiScreen IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.

Caratteristiche e vantaggi

  • DEAE Sepharose Fast FLow is a weak anion exchanger and IEX Sepharose Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
  • Prepacked, ready-to-use columns for convenient process development optimization.
  • 10 cm bed height of HiScreen columns is designed to allow method optimization and parameter screening.
  • Easily connected in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption.
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Prodotti correlati

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Pittogrammi

Flame

Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


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Articoli

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocolli

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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