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Documenti fondamentali

GE17-5168-01

Sigma-Aldrich

Mono S® 5/50 GL

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About This Item

Codice UNSPSC:
41115700
NACRES:
SB.52

ligand

-OCH2CH(OH)CH2OCH2CH(OH)CH2SO3-

Materiali

PEEK column

Descrizione

Ion Exchanger Type (Methyl sulfonate strong cation)

Confezionamento

pkg of 1 mL

Parametri

580 psi

Dimensioni del letto

5 mm × 50 mm

Volume del letto

1 mL

Diam. int. colonna

5.7 mm

Matrice

monodisperse porous polystyrene/divinyl benzene beads
polymeric

Gruppo funzionale matrice

methyl sulfonate phase

Dimens. media delle particelle

10 μm

cleaning

1-14

Intervallo di esercizio

2-12

Tecnica di separazione

cation exchange

Descrizione generale

Mono S® is a strong cation exchanger prepacked with MonoBeads in a Tricorn column. The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required. The column has 1 mL bed volume for small-scale purifications.

The Tricorn columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads columns.

Caratteristiche e vantaggi

  • Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
  • Compatible with AKTA design, FPLC™ System, and other high-performance LC systems.
  • MonoBeads are also available as Mono P® for chromatofocusing

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Prodotti consigliati

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Note legali

FPLC is a trademark of Cytiva
Mono P is a registered trademark of Cytiva
Mono S is a registered trademark of Cytiva

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

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Certificati d'analisi (COA)

Lot/Batch Number

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Articoli

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This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Vedi tutto

Protocolli

MonoBeads: Purification of Milligram Quantities with Highest Resolution

This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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