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Documenti fondamentali

GE11-0032-75

HiTrap® Capto™ MMC

Cytiva 11-0032-75, pack of 5 × 5 mL

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About This Item

Codice UNSPSC:
23151817
NACRES:
NA.56

Descrizione

Ion Exchanger Type (value)

Confezionamento

pack of 5 × 5 mL

Produttore/marchio commerciale

Cytiva 11-0032-75

Parametri

<20 ml/min flow rate
42 psi

Dimensioni del letto

16 mm × 25 mm

Volume del letto

5 mL

Diam. int. colonna

16 mm

Matrice

highly cross-linked agarose

Dimens. media delle particelle

75 μm (d50v)

cleaning

2-14

Intervallo di esercizio

2-12

Compatibilità

suitable for bioprocess medium

Descrizione generale

HiTrap® Capto™ MMC is prepacked with Capto™ MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.

Applicazioni

The multimodal functionality gives a different selectivity compared with traditional ion exchangers including binding of Proteins regardless of ionic strength of the loading material . This means that the medium may be used for direct load of clarified feedstocks, without prior dilution to reduce the conductivity of the starting material. To reduce tedious sample preparation, the top filter in HiTrap® Capto™ MMC has been optimized for loading sonicated unclarified cell lysate directly on the column.

The Capto™ MMC ligand may interact with Proteins in many different ways. It contains a carboxyl group, and thus it is a weak cation exchanger. However, in addition to the ionic interactions several other types of interactions are involved, including hydrogen bonding and hydrophobic interaction.

Caratteristiche e vantaggi

  • High dynamic binding capacity at high conductivity.
  • Selectivity allows binding in the presence of salt.
  • High volume throughput.
  • HiTrap® Capto™ MMC can be used to bind Proteins at the conductivity of the feed material and to solve specific purification challenges.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

Capto is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva

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Indicazioni di pericolo

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Codice della classe di stoccaggio

3 - Flammable liquids

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Certificati d'analisi (COA)

Lot/Batch Number

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Protocolli

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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