G115000

DMT-dG(dmf) Phosphoramidite

≥99.0% (reversed phase HPLC)

• Sinonimo/i: DMT-dG(dmf) Amidite, N-[(dimethylamino)methylene]-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxyguanosine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite], N2-Dimethylformamidine-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine-3′-O-[O-(2-cyanoethyl)-N,N′-diisopropylphosphoramidite]
• Formula empirica (notazione di Hill): C₄₃H₅₃N₈O₇P
• Numero CAS: 330628-04-1
• Peso molecolare: 824.90
• Numero MDL: MFCD00797420
• Codice UNSPSC: 12352200
• ID PubChem: 329799820
• NACRES: NA.51

Proprietà

• Tipo: for DNA synthesis
• Livello qualitativo: 200
• Nome Commerciale: Proligo Reagents
• Saggio: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• Forma fisica: powder
• PM: 824.90 g/mol
• tecniche: oligo synthesis: suitable
• Colore: white to off-white
• λ: conforms (UV/VIS Identity)
• Profilo del nucleoside: base: deoxyguanosine; base protecting group: DMF; 2' protecting group: none; 5' protecting group: DMT; deprotection: fast
• Temperatura di conservazione: -10 to -25°C
• Stringa SMILE: COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(\N=C/N(C)C)=Nc34)(c5ccccc5)c6ccc(OC)cc6
• InChI: 1S/C43H53N8O7P/c1-29(2)51(30(3)4)59(56-24-12-23-44)58-36-25-38(50-28-45-39-40(50)47-42(48-41(39)52)46-27-49(5)6)57-37(36)26-55-43(31-13-10-9-11-14-31,32-15-19-34(53-7)20-16-32)33-17-21-35(54-8)22-18-33/h9-11,13-22,27-30,36-38H,12,24-26H2,1-8H3,(H,47,48,52)/b46-27-/t36-,37+,38+,59?/m0/s1; YRQAXTCBMPFGAN-UJASEYITSA-N

Descrizione

Descrizione generale

DMT-dG(dmf) Phosphoramidite belongs to fast deprotection phosphoramidites where the deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. Changing the dG protecting group to the dimethylformamidine (dmf) baseprotecting group enables the rapid synthesis of high-purity, high-yield oligonucleotide, thus increasing the efficiency of high-throughput production.

Caratteristiche e vantaggi

Key Features of dG(dmf):

• dG(dmf) is deprotected faster than the conventional dG(ib): the deprotection time in concentrated ammonia is reduced to 2 hours at 55 °C or 1 hour at 65 °C
• The dG(dmf)-monomer is especially suitable for G-rich sequences: incomplete deprotection is greatly reduced in comparison with the conventional dG(ib)-monomer
• dG(dmf)-phosphoramidite is as stable in solution as the standard dA(bz)-, dC(bz)- and dT-phosphoramidites
• dG(dmf)-phosphoramidite can directly substitute for dG(ib)-phosphoramidite
• No change is required in the reagents commonly used for DNA synthesis (Except a low concentration iodine oxidizer i.e., 0.02 M in iodine, should be employed)

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable