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F4137

Sigma-Aldrich

Anti-Chicken IgY (IgG) (whole molecule)−FITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sinonimo/i:

Rabbit Anti-Chicken IgY (IgG) (whole molecule)−Fluorescein isothiocyanate

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

rabbit

Livello qualitativo

Coniugato

FITC conjugate

Forma dell’anticorpo

IgG fraction of antiserum

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Stato

buffered aqueous solution

Condizioni di stoccaggio

protect from light

tecniche

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
indirect immunofluorescence: 1:200

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

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Descrizione generale

Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa.
Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus chicken antibodies are useful analytical tools for various research applications .

Specificità

Birds, reptiles and amphibians express IgY antibodies that are functionally equivalent to IgG. Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for IgY of chicken. Rabbit Anti- Chicken IgY (IgG) is conjugated to fluorescein isothiocyanate (FITC). Free FITC is removed by gel filtration.

Immunogeno

Chicken IgY (IgG)
Purified chicken IgY (IgG)

Applicazioni

Anti-Chicken IgY (IgG) (whole molecule) FITC antibody has been used:
  • in indirect immunofluorescence
  • in western blotting
  • in immunocytochemistry

Azioni biochim/fisiol

IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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IgY is the functional equivalent of IgG in birds, reptiles and amphibia, but many aspects of its biology are poorly understood. Recent studies have increased awareness of the genetics and functions of this molecule, and have revealed its position as

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