E0521

Anti-Estrogen Receptor (18-32) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Numero MDL: MFCD00676446
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: IgG fraction of antiserum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• PM: antigen 67 kDa
• Reattività contro le specie: human
• tecniche: microarray: suitable; western blot: 1:3000 using MCF7 cultured cell line extract
• N° accesso UniProt: P03372
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ESR1(2099)

Descrizione

Descrizione generale

Estrogen is a sex hormone that is a key regulator of growth, differentiation and function of reproductive tissues, skeletal and cardiovascular systems. The effect of estrogen is mediated by two intracellular receptors, ERα and ERβ. Although the two receptors interact with similar ligands, have identical DNA-binding domains and have redundant roles in estrogen signaling, they show distinct expression pattern. On binding to the receptor, the estrogen-ER complex enters nucleus and regulates the transcription of specific genes. The biological effects of estrogen and ER is by upstream activation of the pathway by growth factors. Some pathways that activate the estrogen-ER pathway are those mediated by AP-1, IGF-1, EGF, MAPK and PI3K/Akt . Anti-Estrogen Receptor (18-32) antibody is specific for 18-32 amino acids of human estrogen receptor (67 kDa). The product is an IgG fraction prepared from pooled antisera.

Immunogeno

synthetic peptide corresponding to amino acids 18-32 of the human estrogen receptor.

Applicazioni

Anti-Estrogen Receptor (18-32) antibody may be used for immunoblotting using extracts of MCF7 cells at a working dilution of 1:3000. It may also be used for dot blot.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)