D8062

DMEM/F-12

With ʟ-glutamine, sodium bicarbonate and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

• Sinonimo/i: DME/F-12, miscela 1:1
• Numero MDL: MFCD00217417
• Codice UNSPSC: 12352207
• NACRES: NA.75

Proprietà

• Nome del prodotto: Terreno Eagle modificato di Dulbecco/miscela nutritiva Ham F-12, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture
• Livello qualitativo: 400
• Sterilità: sterile-filtered
• Stato: liquid
• tecniche: cell culture | mammalian: suitable
• Impurezze: endotoxin, tested
• Componenti: glucose: 3.15 g/L; NaHCO₃: yes; L-glutamine: yes; phenol red: yes; HEPES: no; sodium pyruvate: 0.055 g/L
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Media are supplemented with a defined mixture of nutrients, growth factors, and hormones instead of serum. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used for these defined studies. To compensate for the loss of buffering capacity caused by eliminating serum, a final concentration of 15 mM HEPES buffer can be added to the formulations. It allows the investigator to optimize the buffering system.

Applicazioni

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

• to maintain human lung and colorectal cancer cell lines for the biological evaluation of the secondary metabolites
• to culture human kidney-2 (HK-2) cells and as a component of the assay medium for lysophosphatidylcholine (LPC) treatment
• to culture mouse squamous cell carcinoma (SCCVII) tumor cells

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable