D2196

DAF-FM solution

5 mM in DMSO, ≥97% (HPLC)

• Sinonimo/i: 4-Amino-5-methylamino- 2′,7′-difluorofluorescein, Diaminofluorescein-FM
• Formula empirica (notazione di Hill): C₂₁H₁₄F₂N₂O₅
• Numero CAS: 254109-20-1
• Peso molecolare: 412.34
• Codice UNSPSC: 12352200
• NACRES: NA.77

Proprietà

• Livello qualitativo: 100
• Saggio: ≥97% (HPLC)
• Forma fisica: DMSO solution
• Condizioni di stoccaggio: desiccated
• Colore: colorless to yellow, including pink
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• InChI: 1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3; DIJCILWNOLHJCG-UHFFFAOYSA-N

Descrizione

Applicazioni

DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.

Azioni biochim/fisiol

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm; Em: 515 nm; dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.

Caratteristiche e vantaggi

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable