CRISPRPL02
CRISPR GUS GAPDH Reporter Control for Dicots
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About This Item
Prodotti consigliati
Ricombinante
expressed in E. coli
Livello qualitativo
Confezionamento
vial of 50 μL
Concentrazione
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
applicazioni
CRISPR
selection
kanamycin
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Descrizione generale
All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids with GUS Reporter
CRISPR Plant Cas9 products are intended for Agrobacterium-mediated plant transformation. The products are based on the type IIA CRISPR-Cas9 derived from Streptococcus pyogenes. The native Cas9 coding sequence is codon optimized for expression in monocots and dicots, respectively. The monocot Cas9 constructs contain a monocot U6 promoter for sgRNA expression, and the dicot Cas9 constructs contain a dicot U6 promoter.
Arabidopsis seedlings were germinated in 6 well tissue culture plates
The seedlings were infected with Agrobacterium which had the CRISPR plasmids with a GUS reporter. After 3-4 days of transfection the GUS expression was detected. b-glucuronidase (GUS) is an enzyme that hydrolyzes colorless glucuronides to yield colored product
CRISPR Plant Cas9 products are intended for Agrobacterium-mediated plant transformation. The products are based on the type IIA CRISPR-Cas9 derived from Streptococcus pyogenes. The native Cas9 coding sequence is codon optimized for expression in monocots and dicots, respectively. The monocot Cas9 constructs contain a monocot U6 promoter for sgRNA expression, and the dicot Cas9 constructs contain a dicot U6 promoter.
Arabidopsis seedlings were germinated in 6 well tissue culture plates
The seedlings were infected with Agrobacterium which had the CRISPR plasmids with a GUS reporter. After 3-4 days of transfection the GUS expression was detected. b-glucuronidase (GUS) is an enzyme that hydrolyzes colorless glucuronides to yield colored product
Applicazioni
- To verify successful integration of T-DNA in plant genome
- GUS receptor wheat gGAPDH control for monocots for Agrobacterium mediated transformation
Caratteristiche e vantaggi
- Low cost, genome editing option compared to other methods.
- Easy to use
- Online ordering
- Ready to ship in 2 days
Componenti
1 vial containing 50ul of 20ng/ul plasmid DNA
Keep reagent tubes closed when not in use.
Practice aseptic lab technique to avoid DNase contamination.
Keep reagent tubes closed when not in use.
Practice aseptic lab technique to avoid DNase contamination.
Principio
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
Altre note
For ordering any of our custom CRISPR plant products please visit: CUSTOM ORDERING FORM
Note legali
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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