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CMC0022

Sigma-Aldrich

OverExpress™ C43(DE3) Electrocompetent Cells

for the highest protein expression

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.85

Origine biologica

Escherichia coli

Grado

for molecular biology

Modalità di accrescimento

adherent or suspension

Morfologia

rod shaped

tecniche

microbiological culture: suitable

Trasformazione cellulare

competent cell type: electrocompetent
transformation efficiency: ≥1 x 109 cfu/μg

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Descrizione generale

OverExpress™ Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress™ strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress™ C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Caratteristiche e vantaggi

  • Express genes cloned into any T7 vector with these BL21(DE3) derivatives
  • Effective in expressing toxic & membrane proteins
  • Cited in over 350 research articles

Componenti

  • OverExpress C43(DE3) electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression

Note legali

OverExpress is a trademark of Imaxio, S.A.

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Codice della classe di stoccaggio

10 - Combustible liquids

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A Rangunwala et al.
Epidemiology and infection, 142(10), 2147-2154 (2013-12-18)
Crimean-Congo haemorrhagic fever virus (CCHFV) has the propensity to cause nosocomial infections with a high fatality rate. Handling the virus requires biosafety level-4 facilities, limiting accessibility for many laboratories. Advances in molecular techniques have allowed preparation of safe recombinant antigens
Arthur Laganowsky et al.
Nature, 510(7503), 172-175 (2014-06-06)
Previous studies have established that the folding, structure and function of membrane proteins are influenced by their lipid environments and that lipids can bind to specific sites, for example, in potassium channels. Fundamental questions remain however regarding the extent of

Protocolli

OverExpress™ Electrocompetent Cells

OverExpress™ Electrocompetent Cells are provided in 25 μL aliquots, sufficient for one transformation reaction.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.