MEF-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 98061101, mouse embryo, Fibroblast-like

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: mouse embryo
• Confezionamento: tube of 5 μg 98061101-DNA-5UG; pkg of vial of cells 98061101-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Fibroblast-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse embryo fibroblast, SV40 - transformed

Descrizione della linea cellulare

The cell line MEF-1 was established by transfection of mouse embryo fibroblasts ((C57 BL/6 x 129) F1) with a SV40 coding plasmid. MEF-1 express the wild-type for low density lipoprotein receptor related protein (LRP). Together with the cell lines PEA10 (Sigma Catalogue number. 97040310) and PEA13 (Sigma Catalogue number. 97040311), which express heterozygous and homozygous forms of LPR, an experimental system is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LPR - deficiency. This is of importance since LRP - deficient mouse embryos die early during gestation.

Applicazioni

Study of LRP and LRP-deficiency

Terreno di coltura

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:6 to 1:10 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Altre note

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