MFE-296

98031101, human endometrium, Epithelial

• Sinonimo/i: MFE296 Cells
• Codice UNSPSC: 41106514

Proprietà

• product name: MFE-296, 98031101
• Origine biologica: human endometrium
• Modalità di accrescimento: Adherent
• Cariotipo: Near Tetraploid
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Androgen receptor
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human caucasian endometrial adenocarcinoma

Descrizione della linea cellulare

MFE-296 was obtained from a moderately differentiated human endometrial adenocarcinoma. The cells were shown to express functionally intact androgen receptors. Proliferation was inhibted by progestin R5020 and dihydrotestosterone (DHT), the latter one being antagonised by the antiandrogen Casodex. MFE-296 express vimentin and the cytokeratins 7, 8, 18 and 19. Karyotype analysis revealed near tetraploidy for most cells. Further more, the cells have been shown to be tumorigenic in nude mice.

Applicazioni

Tumour biology studies, receptor analysis

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,13
D16S539: 11,14
D5S818: 11,13
D7S820: 8,10
THO1: 6,9.3
TPOX: 8
vWA: 14,18

Terreno di coltura

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding 1-2 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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