PG-4

94102703, feline brain, Glial/astrocyte

• Codice UNSPSC: 41106514

Proprietà

• product name: PG-4, 94102703
• Origine biologica: feline brain
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Glial/astrocyte
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Cat brain Moloney sarcoma virus-transformed

Descrizione della linea cellulare

PG-4 was derived at NIH in 1980 from G355 cells by transformation with Moloney Murine sarcoma virus (M-MST) and subsequently cloned. Support the growth of simian sarcoma associated virus (SSAV); macaque virus; feline immunodeficiency virus (FeLV) A, B and C; feline RD-114; C retroviruses; murine xenotropic and munk cell focus-inducing virus (MCF).

Applicazioni

Cells can be used to detect and assay type C retroviruses and are currently recommended by the FDA for the detection of replication competent retroviruses in gene therapy products and hybridomas producing reagents for human use.

PG-4 cells can be used to detect and assay type C retroviruses and are currently recommended by the FDA for the detection of replication competent retroviruses in gene therapy products and hybridomas producing reagents for human use.

Terreno di coltura

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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