DSL6A/C1

94022431, rat pancreas, Polygonal

• Codice UNSPSC: 41106514

Proprietà

• product name: DSL6A/C1, 94022431
• Origine biologica: rat pancreas
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Polygonal
• Prodotti: Expresses CFTR protein
• Recettori: Lacks CCK-A receptors
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat pancreatic carcinoma

Descrizione della linea cellulare

DSL6A/C1 were derived from a transplantable acinar cell carcinoma (DSL-6) that had been established from a primary carcinoma of the pancreas in an azaserine treated Lewis rat. The expression of the ductal cell marker OC.2 was shown with a sub-population expressing ductal marker OV-6, CK-7 and CK-19. DSL6A/C1 cells are reported to be tumorigenic in Lewis rats and produce solid tumours composed of duct like structures surrounded by dense fibrous tissue. The cell line lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and re-grafting.

Applicazioni

Study of pancreatic carcinomas, differentiation of acinar/ductal cells.

Terreno di coltura

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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