C2C12 Cell Line from mouse

91031101, mouse muscle, Myoblast

• Sinonimo/i: C2-C12 Cells
• Codice UNSPSC: 41106514

Proprietà

• product name: C2C12 Cell Line from mouse, C3H muscle myoblast, 91031101
• Origine biologica: mouse muscle
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Myoblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse C3H muscle myoblast

Descrizione della linea cellulare

Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.

Applicazioni

C2C12 Cell Line has been used to study the the expression of OXT and OXTR (oxytocin and its receptor) genes and proteins and in differentiation studies.

Terreno di coltura

DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum FBS / FCS.

Mantenimento delle subcolture

Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C. Do not allow cells to reach confluence.

Altre note

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