GS-109-V-20

90110505, human skin, Fibroblast-like

• Codice UNSPSC: 41106514

Proprietà

• product name: GS-109-V-20, 90110505
• Origine biologica: human skin
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 46
• Morfologia: Fibroblast-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian skin fibroblast Gardner′s syndrome

Descrizione della linea cellulare

Derived from skin from the upper arm of an 18 year old Caucasian male with Gardner′s syndrome is an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. Cells exist in an initiated state rather than a normal or transformed state. Normal diploid karyotype.

Applicazioni

Tumourigenicity studies

Terreno di coltura

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Monolayer will form in a 25cm² flask in 1 week.

Altre note

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